The 67th JSAP Spring Meeting 2020

Presentation information

Poster presentation

3 Optics and Photonics » 3.4 Biomedical optics

[14a-PB2-1~15] 3.4 Biomedical optics

Sat. Mar 14, 2020 9:30 AM - 11:30 AM PB2 (PB)

9:30 AM - 11:30 AM

[14a-PB2-13] Cell cycle dependence of phototoxicity of living cells irradiated
by 450 nm laser light

Mikiya Yamaguchi1, Naohiro Kanamaru1, Tetsuya Matsuyama1, Kenji Wada1, Koichi Okamaoto1, Ai Kawawakita2, Kaori Murata2, Kenji Sugimoto2 (1.Engineering Osaka Pref.Univ., 2.Life and Environmental Sciences Osaka Pref.Univ.)

Keywords:blue light, phototoxicity, PCNA

In live cell imaging, we showed that blue light causes DNA damage in living cells by observing the dynamics of proliferating cell nuclear antigen (PCNA) after irradiation with blue laser light. Laser light was applied to living cells in the M and S phases to examine the cell cycle dependence of phototoxicity. These results indicate that in the S phase, PCNA accumulates at the site irradiated with laser light to repair DNA damage, and the action of PCNA causes cell cycle dependence of phototoxicity.