[14p-B410-1~7] Fundamental and applications of spatial light modulation

[14p-B410-2] Fluorescence microscopy using spatial light modulation

〇Osamu Matoba¹, Xiangyu Quan¹, Manoj Kumar¹, Yasuhiro Awatasuji² (1.Kobe Univ., 2.Kyoto Inst. Tech.)

Keywords:Fluorescence holography, Digital holography

Fluorescence digital holography that can measure a three-dimensional fluorescence distribution in a single recording is introduced. In fluorescence digital holography, spatially spread fluorescence light wave as the low-coherent light is modulated by a lens function and a diffraction grating displayed on a phase-mode spatial light modulator and then is recorded in an image sensor after making an interference pattern with an unmodulated light wave. The cross-sectional images are reconstructed numerically by numerical coherent wave propagation calculation. In the presentation, we describe the experimental results using plant cells and then the future works are discussed.