The 82nd JSAP Autumn Meeting 2021

Presentation information

Oral presentation

12 Organic Molecules and Bioelectronics » 12.6 Nanobiotechnology

[10p-N207-1~16] 12.6 Nanobiotechnology

Fri. Sep 10, 2021 1:30 PM - 6:00 PM N207 (Oral)

Koji Sumitomo(Univ. of Hyogo), Hitoshi Asakawa(Kanazawa Univ.), Akahori Rena(日立研開)

5:30 PM - 5:45 PM

[10p-N207-15] Control of Vesicle Fusion by Biotin-Streptavidin interaction

Daiya Mombayashi1, Kai Hashino1, Azusa Oshima2, Masumi Yamaguchi2, Akira Heya1, Koji Sumitomo1 (1.Univ. of Hyogo, 2.NTT Basic Res. Labs., BMC)

Keywords:vesicle fusion, FRAP

Vesicles were tethered with the supported lipid bilayer (SLB) by Biotin-Streptavidin interaction, and the fusion state was investigated by Fluorescence Recovery After Photobleaching (FRAP).When the addition of Ca2+ ions in the buffer solution suppressed the electrostatic repulsion between vesicles and the SLB, the mobile fraction increased and it indicates that fusion proceeds.In the case of the vesicles composed of DOPC / DOPE, the structural stability is low, so fusion is facilitated even in the absence of Ca2+ ions.