[10p-N207-1~16] 12.6 Nanobiotechnology

[10p-N207-4] Nanopore devices for exosomes

〇Sou Ryuzaki^1,2, Makusu Tsutsui³, Takao Yasui⁴, Yoshinobu Baba⁴, Masateru Taniguchi³ (1.Kyushu Univ., 2.JST PRESTO, 3.Osaka Univ., 4.Nagoya Univ.)

Keywords:nanopore, exosome, cancer

A rapid and simple cancer detection method independent of cancer type is an important technology for cancer diagnosis. Although the expression profiles of biological molecules contained in cancer cell-derived extracellular vesicles (EVs) are considered candidates for discrimination indexes to identify any cancerous cells in the body, it takes a certain amount of time to examine these expression profiles. Here, we report the shape distributions of EVs suspended in a solution and the potential of these distributions as a discrimination index to discriminate cancer cells. Distribution analysis is achieved by low-aspect-ratio nanopore devices that enable us to rapidly analyze EV shapes individually in solution, and the present results reveal a dependence of EV shape distribution on the type of cell (cultured liver, breast, and colorectal cancer cells and cultured normal breast cells) secreting EVs. The findings in this study provide a realizability and experimental basis for a simple method to discriminate several types of cancerous cells based on rapid analyses of EV shape distributions.