[12a-S402-1~10] 12.6 Nanobiotechnology

[12a-S402-4] Detection of marker molecules expressed on cancer cell surfaces based on electrochemiluminescence measurements

Kohki Uchiyama^1,2, Airi Kuriyama², Tomoyuki Kamata³, Dai Kato³, Naoshi Kojima³, Shohei Yamamura³, 〇Hyonchol Kim^1,4 (1.Cell. Mol. Biotechnol. Res. Inst., AIST, 2.Fac.. Eng., Tokyo Univ. Agric. Technol., 3.Health Med. Res. Inst., AIST, 4.Grad. Sch. Eng., Tokyo Univ. Agric. Technol.)

Keywords:Cancer cells, Electrochemiluminescence, Cell measurement

Biomarker molecules expressed on cell surfaces were successfully detected by using a new method based on electrochemiluminescence (ECL) observation. Cancer cells on which target molecules, EpCAMs, were a few expressed were reacted with ECL probe-conjugated anti-EpCAM antibodies, and were captured to cup-shaped microelectrodes. The EpCAM molecules were detected by the irradiation of ECL with an application of electric voltage to the cup electrodes.