[13a-N107-1~12] 3.1 Basic optics and frontier of optics

[13a-N107-1] Three-Dimensional Imaging by Light Needle Scanning Fluorescence Microscopy Utilizing Visible Bessel Beams

〇Yuichi Kozawa¹, Yuuki Uesugi¹, Sato Shunichi¹ (1.IMRAM, Tohoku Univ.)

Keywords:Bessel beam, Laser microscopy, 3D imaging

We demonstrate a needle-shaped focal spot with an extended focal depth, characterized by reduced side lobes along the specific axis on the beam cross-section achieved by manipulating the intensity distribution of a Bessel beam formed at the focus of an objective lens. This property is applied to our three-dimensional imaging technique based on light needle scanning microscopy, enabling rapid acquisition of three-dimensional fluorescence images obtained by one-photon absorption using visible excitation.