[13a-N322-1~13] 12.7 Biomedical Engineering and Biochips

[13a-N322-8] Anomalous Enhancement of Charge Transfer between Hexacyanoferrate Ions and Carbonenous Electrodes by Ru Complex Ion

〇Ichiro Yamashita¹, Huanwen Han¹, Kazuyuki Nobusawa^1,2 (1.Osaka Univ., 2.Textile Res. Inst. Gunma)

Keywords:Electrochemical, PCR, Electrochemical Impedance spectroscopy

We are developing a new electrochemical impedance measurement method for real-time detection of the polymerase chain reaction (PCR). The basis of the method is the phenomenon that the synthesized DNA intercalator [Ru(bpy)₂DPPZ]²+ promotes charge transfer between hexacyanoferrate ions and carbonaceous electrodes in PCR solution at a concentration of only 1 µM. It was experimentally confirmed that the effect is generated by the hexacyanoferrate ion, the [Ru(bpy)2DPPZ]2+ ion, and the carbonaceous electrode, and is independent of other ions such as electrolyte ions.