The 82nd JSAP Autumn Meeting 2021

Presentation information

Oral presentation

1 Interdisciplinary Physics and Related Areas of Science and Technology » 1.5 Instrumentation, measurement and Metrology

[13p-S401-1~11] 1.5 Instrumentation, measurement and Metrology

Mon. Sep 13, 2021 1:00 PM - 3:45 PM S401 (Oral)

Nao Terasaki(AIST)

2:30 PM - 2:45 PM

[13p-S401-7] Development of a whole neural network tracking system for real-time high-resolution light-field imaging in freely behaving C. elegans.

Haruka Maeoka1, Shigyou Kazuki1, Sugi Takuma1 (1.Hiroshima Univ.)

Keywords:fluorescent imaging, image analysis, cell tracking

How does the spatiotemporal pattern of brain networks decline in the aging process? Understanding such patterns requires a method for quantifying neural activities noninvasively and repeatedly throughout life-course. Here, we are developing a system to quantify a whole-brain activity in freely behaving nematode C. elegans. We developed the software that extracts fluorescence of a calcium indicator G-CaMP expressed in all neurons of worms. Then, this software controls the motorized stage to keep all neurons of a worm under the field of view of the microscope. Combining this tracking system with our developed high-resolution light-field microscopy for real-time, scan-less imaging will allow for examining the spatiotemporal pattern of an aging neural network at single-cell resolution in the future.