[16a-Z22-1~10] 12.7 Biomedical Engineering and Biochips

△ [16a-Z22-4] Microfluidics of single-cell level analysis for antigen-specific T cell detection

〇Hiroki Ide^1,2, Masato Saito^1,2, Taiki Aoshi³, Hyota Takamatsu⁴, Wilfred Espulgar¹, Masahito Hosokawa⁵, Hiroko Matsunaga⁵, Naoko Suzuki⁵, Koji Arikawa⁵, Haruko Takeyama^5,6,7, Eiichi Tamiya^2,8 (1.Graduate School of Engineering Osaka Univ., 2.AIST-Osaka Univ. PhotoBIO-OIL, 3.Research Institute for Microbial Disease, Osaka Univ., 4.Graduate School of Medicine, Osaka Univ., 5.Research organization for Nano and Life Innovation, Waseda Univ, 6.Department of Life Science and Medical Bioscience, Waseda Univ., 7.CBBD-OIL, AIST-Waseda Univ., 8.The Institute of Scientific and Industrial Research, Osaka Univ.)

Keywords:Microfluidics, Single-cell analysis

Immune responses are elicited by T cells that specifically interact with antigen-presenting cells (APCs). T cell receptor (TCR) recognizes a peptide derived from a pathogen presented by APC and then T cell immunologically activates. However, the recognition is very rare event due to the genomic rearrangement of the germline on TCR locus. It makes the analysis of T cell activation which elicits immune responses a challenge by using conventional methods. Thus, a new platform is required to promote, detect and single-cell-level measure T cell behavior in paring. In this study, we developed a device for the formation of T cell-APC pairs at the single cell level and profiling each T cell activation based on intracellular Ca²⁺ flux as an indicator. In addition, we collected T cells after the measurement and analyzed by single cell RNA-Seq to link the intracellular Ca²⁺ signal and T cell gene expression.