[16p-Z12-1~17] 8.8 Division of Plasma Electronics 30th Anniversary Special Session

[16p-Z12-15] Examination of usefulness of cell medicine using plasma gene transfer method

〇(B)Kenjirou Onishi¹, Akiho Wakamura², Satomi Ihara⁴, Susumu Satou³, Masafumi Zinno² (1.Ehime Univ., 2.Ehime Univ, 3.i-Gene, 4.Preal Kogyo)

Keywords:plasma medical

We investigated the possibility of cell medicine by plasma gene transfection method using a mouse type I diabetes model. Insulin gene coded plasmid is transferred into cell by micro plasma treatment and is expressed in mouse hepatocytes and blood cells. Moreover, the random integration of the transgene into the genome was very low compared to existing methods such as electroporation. These results are suggested that the micro plasma gene transfection method enables practical application such as cell medicine.