[17a-P09-1~7] 12.7 Biomedical Engineering and Biochips

▲ [17a-P09-3] Efficient PDMS through-hole membrane fabrication
for microwell-based bioassay utility

〇(PC)Wilfred Espulgar¹, Masato Saito¹, Shohei Koyama¹, Hyota Takamatsu¹, Eiichi Tamiya¹ (1.Osaka Univ.)

Keywords:microfluidics, microwell, bioassay

A variety of miniaturized and microwell devices have shown useful in many chemical and biological assays. Miniaturization allows low volume sampling at a high number of tests per area that enables to test as many compounds as possible from a raw sample. PDMS (Polydimethylsiloxane) based microwells has been used in many applications as well; however, many issues such as restricted chemical compatibility, swelling when in contact with many common chemicals, shrinking when ambient humidity is low, and readily partition of small molecules from the bulk PDMS can complicate the on-chip concentration and may invalidate the measurement. To address this problem, a thin PDMS through-hole membrane has been realized. A thin PDMS can minimize absorption and having a glass bottom can ensure easy surface modification for a variety of assay applications. The conventional mechanical press, PDMS between weight and SU-mold, has been utilized for fabrication where A COP film coated with PVA was used as a carrier film.