[19p-Z17-1~17] 8.4 Plasma life sciences

[19p-Z17-2] Analysis of effects caused by non-thermal atmospheric pressure plasma on iPS cell differentiation

〇Mime Kobayashi¹, Kiichiro Tomoda^1,2, Tetsuji Shimizu³, Michio Asahi¹, Shinya Kumagai⁴ (1.Osaka Med. College, 2.Gladstone Inst., 3.AIST, 4.Meijo Univ. Dept. of Eng.)

Keywords:cell, atmospheric pressure plasma, dielectric barrier discharge

Non-thermal atmospheric pressure plasma generates active species such as radicals, ions, and electrons. Plasma irradiation has been used for biological applications, including the selective killing of cells and enhancement of plant growth. Human induced pluripotent stem cells (hiPSCs) can differentiate into any types of cells while infinitely proliferate in vitro. Given plasma affects a broad range of biological events, it may also enhance differentiation or proliferation of hiPSCs. In this research, hiPSCs were treated with dielectric barrier discharge (DBD) air plasma (9 kV, 12.5 kHz) to gain insights into plasma applications. Plasma was generated in the atmosphere at room temperature. Our results sugest that differentiation toward mesoderm is prefered at one plasma irradiation condition used. At the same time, plasma did not affect survival of DNA-repair-deficient cells indicating that the treatment do not damage chromatin DNA at the condition used. Immunohistochemical staining using E-cadherin antibody suggests that plasma irradiation influence cell-cell attachment. Detailed mechanisms behind plasma effects will be revealed by RNA expression analyses. References: Kumagai et al. (2020), Electron. Comm. Jpn. 103, 43; Kumagai et al. (2016) Jap. J. Appl. Phys. 55, 01AF01; Kobayashi et al. (2016) Appl. Phys. Express 9, 127001; Kime et al. (2019) Stem Cell Rep. 13, 485.