[20a-P06-1~11] 12.7 Biomedical Engineering and Biochips

[20a-P06-3] Improvement of gene transfer efficiency in cell establishment with higher safety for gene therapy by using surface discharge

〇Kenjirou Onishi¹, Ryota Tamura¹, Satomi Ihara³, Yoshihisa Ikeda¹, Susumu Satoh^1,2, Masafumi Jinno^1,2 (1.Ehime Univ., 2.i-Gene Corporation, 3.Pearl Kogyo Co)

Keywords:plasma gene transfer, surface discharge, gene therapy

We investigate the easy method of safe cell establishment for gene therapy using surface discharge. The experimental results show that gene transferred cells efficiently express target protein and suggest that surface discharge is an efficient gene transfer method for primary cells. In addition, we found another essential feature of the surface discharge method. This method induces low/no random integration in the genome of the established cells.