[20a-P06-1~11] 12.7 医用工学・バイオチップ

[20a-P06-3] 細胞医療への応用に向けた安全性の高い沿面放電を用いた遺伝子導入法の効率改善

〇大西 謙二朗¹、田村 亮太¹、庵原 聖未³、池田 善久¹、佐藤 晋^1,2、神野 雅文^1,2 (1.愛媛大学大学院工、2.アイジーン株式会社、3.パール工業)

キーワード：プラズマ遺伝子導入、沿面放電、遺伝子治療

We investigate the easy method of safe cell establishment for gene therapy using surface discharge. The experimental results show that gene transferred cells efficiently express target protein and suggest that surface discharge is an efficient gene transfer method for primary cells. In addition, we found another essential feature of the surface discharge method. This method induces low/no random integration in the genome of the established cells.