[20p-C205-1~12] CS.2 Code-sharing Session of 3.2 & 4.4

▲ [20p-C205-6] [INVITED] Wavefront Engineered Light-Needle Microscopy Utilizing Multiplexed Computer-Generated Holograms

〇Yuichi Kozawa¹, Tomoya Nakamura², Yuuki Uesugi¹, Shunichi Sato¹ (1.IMRAM, Tohoku Univ., 2.SANKEN, Osaka Univ.)

Keywords:Laser scanning microscopy, 3D imaging, Bessel beam

We propose a novel imaging technique that can retrieve the axial information in light needle scanning fluorescence microscopy by introducing a method based on a multiplexed computer-generated hologram applied to fluorescence signals. By this technique, axially resolved three-dimensional images can be obtained from a single two-dimensional raster scanning of a needle spot, which remarkably increases the acquisition speed. We demonstrate real-time and video-rate acquisitions of three-dimensional images for fluorescence specimens. The prompt observation of thicker biological samples is also achieved using our method.