[20p-C301-1~17] 3.3 Biomedical optics (formerly 3.4)

[20p-C301-9] Highly sensitive Raman imaging of biological tissue by remote plasmonic enhancement with a column-structured silica layer

〇Takeo Minamikawa^1,2, Yoshinori Harada², Tetsuro Takamatsu², Yukihiro Morimoto^3,4, Masahiro Kawasaki⁵, Mitsuo Kawasaki⁵ (1.Tokushima Univ., 2.Kyoto Pref. Univ. Med., 3.Ushio Inc., 4.Osaka Univ., 5.Kyoto Univ.)

Keywords:Raman spectroscopy, Plasmonic enhancement, Bioimaging

This study investigated a bioimaging method using remote plasmonic enhanced Raman scattering (RPERS), which does not require contact between the metallic nanostructure and the analyte biological tissue. We constructed an RPERS plate with a silica columnar structure on silver nanoislands and attached a biological tissue to the top of the RPERS plate. As a result, we found that Raman enhancement of more than 10⁴ orders was obtained in biological tissues, even though the distance between the silver nanostructure and the analyte specimen was more than 100 nm.