[21p-B200-1~13] Applied Physics to controll cell fate: Innovation by plasma science and biotechnology

[21p-B200-11] Differentiation of human iPS cells affected by non-thermal atmospheric pressure plasma

〇Mime Kobayashi¹ (1.Osaka Med. Pharm. Univ.)

Keywords:iPS cells, cell differentiation, atmospheric pressure plasma

Human induced pluripotent stem cells (hiPSCs) can differentiate into any types of cells while infinitely proliferate in vitro. Given plasma affects a broad range of biological events, plasma exposure may be used for preferential differentiation of hiPSCs. In this study, cells cultured in 35-mm dishes were exposed to non-thermal atmospheric pressure plasma and its effects were analyzed. As an intermediate during the in vitro differentiation into specialized cell types, a structure called embryoid body (EB) was formed. After cells were cultured under EB forming condition for 7 days, RNA expression was analyzed by qPCR. Expression analysis of mRNAs in EBs from plasma-treated hiPSCs revealed repression of ectoderm gene expression, while algorithm score for mesoderm markers increased. DNA damage assessment using DNA-repair-deficient cells showed that, at the dose we used, plasma treatment may not severely damage the genomic DNA of the hiPSCs, and that the cells can be used for further applications. Our results suggest that plasma treatment can be used for directing pluripotent stem cell fate.