[22p-A404-9~9] 8.6 Plasma Electronics English Session

▲ [22p-A404-9] Role of plasma treatment on radish seeds affects germination, morphology, and biochemical responses

〇(PC)Pankaj Attri¹, Takamasa Okumura¹, Kazunori Koga^1,2, Kunihiro Kamataki¹, N. Yamashita¹, Yuichi Tsukada¹, Naho Itagaki¹, Masaharu Shiratani¹ (1.Kyushu University, 2.Nat. Inst. Nat. Sci.)

Keywords:Plasma agriculture, Electron paramagnetic resonance (EPR) spectroscopy, Reactive oxygen and nitrogen species

The use of atmospheric plasma treatment of seeds benefits over conventional treatments due to short treatment time and low-temperature operations [1,2]. During plasma interacts with the oxygen, nitrogen, water, etc., in air, they produce various radical and non-radical species [3]. It is a general fact that reactive species like reactive oxygen and nitrogen species (RONS) can influence plant growth and development. The increased nitrogen nutrients level influences growth hormones, activation of growth-related gene expression, and other physiological processes to understand the plasma effect on seedling growth [4]. Additionally, the presence of RONS can disrupt redox homeostasis and cause mild oxidative stress in plants during vegetative and reproductive stages.
Electron paramagnetic resonance (EPR) spectroscopy is optimal for interpreting any change in paramagnetic defect centers. The use of free radical species as precursors to identify the changes in the biological systems due to physical or chemical stress. Therefore, we used EPR spectroscopy in this study to detect the changes that occurred in seeds before and after plasma treatment. The weak peak at g = 4.3 represents the signal for Fe³⁺, hyperfine lines belonging to the Mn²⁺ peaks, and an intense sharp rise at g = 2.0 attributed to the semiquinone radical. We suggest that CAP treatment can significantly improve the seedling growth with modification in physical and biochemical analysis.