[23a-A105-1~13] 12.7 Biomedical Engineering and Biochips

△ [23a-A105-11] Development of PVC membrane-based label-free potassium ion image sensor and imaging extracellular responses using acutely sliced brain tissue

〇hideo doi¹, Tomoko Horio¹, Bijay Parajuli², Eiji Shigetomi², Youichi Shinozaki², Yong-Joon Choi¹, Toshiaki Hattori¹, Kazuhiro Takahashi¹, Toshihiko Noda¹, Schuichi Koizumi², Kazuaki Sawada¹ (1.Toyohashi Univ., 2.Yamanashi Univ.)

Keywords:Extracellular K+, Label-free potassium ion image sensor, Polyvinyl chloride

Toward high spatiotemporal imaging of extracellular K⁺([K⁺]o) dynamics in the brain, a 23.55 µm-pitch, 128×128 pixels, label-free K⁺ image sensor was fabricated, in which different thicknesses were deposited by controlling the volumes of PVC membrane solution, and the detection performance was investigated. The deposition condition of the K⁺ sensitive membrane was optimized, for which output variation would be decreased to less than 5% with nearly theoretical sensitivity. Additionally, it was demonstrated that the membrane thickness significantly affects [K⁺]o responses in extracellular imaging using brain tissues.