[23a-E105-1~11] 12.7 Biomedical Engineering and Biochips

[23a-E105-8] Changes of beating interval by stimulating cell aggregates mechanically with Real-Time Feedback control

〇Ayu Sasaki¹, Ryu Kidokoro¹, Shota Nozaki¹, Ryuta Watanabe¹, Kaito Kojima¹, Yuuta Moriyama¹, Toshiyuki Mitsui¹ (1.Aogaku Univ.)

Keywords:biophysics, cardiomyocite

The heart is mainly composed of cardiomyocytes and fibroblasts. Mechanical stress may induce the differentiation of fibroblasts into myofibroblasts then cause heart disease. This differentiation leads to delay local action potentials and arrhythmias. In order to reproduce this phenomenon, we built the system of mechanical stimulation with real-time feedback control. Compared to electrical stimulation, the effect of mechanical stimulation is localized and can reproduce the disease.
Cell suspensions of chick embryo hearts were placed on PDMS to create cell aggregates. Our device was placed in the incubator and stimulate the aggregates. The rhythm of beating was detected by motion capture.
The results showed that inter beat interval (IBI) was different in each phase. We report the IBI of short-term stimulus and long-term. Also, differentiation into myofibroblasts can be detected by α-SMA staining. In this presentation, we report the differentiation by stimulus.