[24p-E104-1~16] CS.8 Code-sharing Session of 12.6 & 12.7

[24p-E104-2] Ultra-sensitive detection of miRNA fragment in exosomes using plasmonic biosensor

Shota Hosoyamada¹, Shinichi Tsukumo^1,2, 〇Taira Kajisa^1,3, Koji Yasutomo^1,2, Takeshi Yasui¹ (1.pLED, Tokushima Univ., 2.Grad.Sch.Med., Tokushima Univ., 3.Interdisciplinary New Science Toyo Univ.)

Keywords:surface plasmon resonance, plasmonics, miRNA

In order to detect trace amounts of cancer markers in body fluids, we aimed to detect miRNAs in a label-free and highly sensitive manner using plasmonic techniques, mainly surface plasmon resonance (SPR). Using a single-stranded LNA-modified gold substrate, SPR measurements were performed in a buffer solution containing suspended gold nanoparticles. In the results, miRNAs were successfully detected from 1 pM.