[24p-P01-1~28] 10 Spintronics and Magnetics(Poster)

[24p-P01-1] Quantitative detection of bacteria utilizing magnetic particle-arrangement

〇Eizo Ushijima¹ (1.IMRA JAPAN)

Keywords:Magnetic article-arrangement, Biosensor, Bacteria

Rapid on-site screening of the pathogenic microorganisms is necessary to minimize the spread of infection. It is expected that portable biosensor devices can be used as a solution for on-site screening. Here, the sensor function of the portable biosensor device required for rapid on-site screening is a high sensitivity, short processing time, and compactness. As such a sensor technology, we are researching and developing biosensors using complementary metal oxide semiconductor (CMOS) integrated circuits. So far, we succeeded in implementing a particle magnetic-adsorption mechanism on a CMOS sensor array and forming a local molecular recognition element reaction field in each channel of the CMOS sensor array by the magnetic particle-arrangement method. Currently, the screening target is set to bacteria, and the performance evaluation of biochemical reaction system to measure the number of bacteria is being carried out, and the usefulness as a system is being verified. We examined whether it was possible to detect quantitatively the pseudo-bacteria (red fluorescent particles with antigen fixed on the surface, particle diameter: 5 µm). We arranged the collecting elements (green fluorescent particles with antibody fixed on the surface, particle diameter: 25 µm) that was adsorbed the pseudo-bacteria in an array. And we observed that one pseudo-bacteria was adsorbed on one collecting element. Hence the prospect of quantitatively detecting bacteria was established.