2:00 PM - 2:15 PM
[25p-E105-3] Analysis of non-thermal atmospheric pressure plasma effects on cell membrane and its contribution in gene transfection
Keywords:non-thermal atmospheric pressure plasma, gene transfection, cell membrane
Non-thermal atmospheric pressure plasma (NTAPP) has been used in biomedical researches. One of its attractive applications is gene transfection for affecting cell fate. Although it is necessary to understand its mechanism for further development of the technique, it has not been clear how NTAPP achieves gene delivery into the cells. To reveal one of the steps of gene delivery, we have observed surface morphology changes of murine fibroblast cells (L929) irradiated with NTAPP by scanning electron microscopy. In this study, to find out an appropriate condition for gene delivery, we analyzed the relationship between surface morphologies and proliferations of the cells irradiated with NTAPP. Analysis of NTAPP effects on introduction of green fluorescent protein (GFP)-coding plasmid and the protein expression in L929 cells will be discussed.