11:30 AM - 11:45 AM
▼ [15a-A305-10] SERS-based detection and interaction of DNA bases with gold nanoparticles
Keywords:Raman, DNA, gold nanoparticles
Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for molecular detection that can be applied to DNA and protein sequencing to achieve a higher sensitivity. Since the Raman signal depends on the orientation of the molecules on the gold surface, understanding the interaction of the DNA bases with gold is a vital step towards enabling SERS-based DNA sequencing. This work investigates the orientation of the DNA bases in the gap between two gold nanoparticles by exploring the changes in the relative size of the Raman peaks according to DNA base concentration and pH. The experimental results were supplemented by performing structural relaxations for adenine using DFT. Finally, single-base sensitivity was verified by performing single-shot Raman measurements at very low adenine concentrations.