[15a-A405-1~8] 3.3 Biomedical optics (formerly 3.4)

▼ [15a-A405-2] Application of bio-speckle on micro-bioassay with plankton Ⅳ

〇(D)Devi Arti Devi¹, Hirofumi Kadono¹, Uma Maheswari Rajagopalan² (1.Graduate School of Science and Engineering, Saitama university, Japan, 2.Dept. Mech, Eng. Faculty of Engineering. Shibaura Institute of Technology, Japan)

Keywords:Micro bioassay, biospeckle, Plankton, Heavy metal, Environment toxicity

Zinc (Zn) is one of the most abundantly found heavy metals in the Earth’s crust and is reported to be an essential micronutrient required for the growth of living beings at low concentration. However, it is toxic at high concentrations. According to a global inventory of trace element 462 to 1,380 × 10⁶ kg/year, Zn are released into the environment by mining and smelting activities.
Our method permits to collective evaluation of the zooplanktons motor ability under different concentration of Zn varying from permissible limit (PL), i.e., 5mg/L (in a drinking water) to 2×, 10×, and 20× times the concentration of PL which is based on the dynamic properties of biospeckle that clearly reflect the movement of scattering particles or plankton.
Recently, a micro bioassay is getting attention to quickly evaluate the toxicity of aquatic environment with very low cost. Earlier, we proposed a new technique of Biospeckle micro-bioassay and demonstrated its validity using zooplankton of paramecium exposed to different pH condition as an environment toxicity [1]. Therefore, in this study, we worked with real environmental toxicity i.e., Zn.