[16a-PA03-1~6] 3.3 Biomedical optics (formerly 3.4)

[16a-PA03-6] Development of a high sensitivity biochemical sensor using SERS effect

〇(B)Yanjun Heng¹, Shion Saito¹, Ryutaro Hirai¹, Ryoma Kumagai¹, Shigenobu Kasai¹, Hirokazu Fukidome², Akira Satou², Taiichi Otsuji², Takashi Uchino¹ (1.Tohoku Inst. Tech., 2.Tohoku Univ.)

Keywords:SERS

We develop highly sensitive surface-enhanced Raman spectroscopy (SERS) substrates using Ag nanoholes. SERS is a sensitive and rapid analytical technique that can detect from single-molecule to virus levels using localized surface plasmon resonance (LSPR). For these reasons, SERS has attracted attention in biosensor applications that allow label-free detection with high specificity. In this study, we investigate the stacked SERS substrates where monolayer graphene is coated on the mica substrates with Ag thin films on the back to improve SERS efficiency and surface hydrophobicity. Then sandwich enzyme-linked immunosorbent assay (ELISA) is fabricated on the SERS substrates to detect bovine interleukin-6 (IL-6), inciting bovine mastitis.