[17a-E302-1~11] 12.6 Nanobiotechnology

▼ [17a-E302-9] Advancements of the X-ray Light Sheet Microscope (II);
An Approach to 3D Super-Resolution by Localization of Single Particles

〇Sierra Dean¹, Hidekazu Takano¹, Yoshiki Kohmura¹, Tetsuya Ishikawa¹ (1.RIKEN SPring-8 Center)

Keywords:Super-Resolution, Light Sheet Microscope, 3D Measurement

We present a technique to overcome conventional diffraction-limited resolution in the in-plane direction to realize nearly isotropic nano-resolution with the use of an ultrathin X-ray light sheet microscope. Automated processing with stochastic optical reconstruction microscopy (STORM) and photo-activated localization microscopy (PALM) are used to create a three-dimensional map of the localization of the excited source, which is used to derive a promising method to discern single particles from multiple particle aggregations.