3:20 PM - 3:30 PM
[IX29-29E] Characterization of milk, feces, and cowshed microbiota in Jersey farms
[Objective] To characterize milk, feces, and cowshed microbiota in Jersey farms in summer and winter.
[Methods] Samples were collected in winter 2017 and in summer 2018 in two Jersey farms. Milk, feces, and blood samples were collected from 10 cows each. Feed, bedding, water, and airborne dust were also collected. Bacterial DNAs were purified and assessed by Miseq platform. Raw sequences were processed by QIIME 1 software. Obtained OTUs with 97% similarity were statistically analyzed using PRIMER 7 and JMP software.
[Results] Fecal microbiota were stable regardless of the farms and sampling times. Pseudomonadaceae, Lactobacillaceae, and Moraxellaceae were predominant in winter milk, whereas Pseudomonadaceae, Staphylococcaceae, and Lactobacillaceae were predominant in summer milk. Milk microbiota in winter was not related with fecal and cowshed microbiota but a relation with cowshed was shown in summer. Staphylococcaceae was also shared between airborne dust and two milk samples at one farm, and Pseudomonadaceae, Staphylococcaceae, and Enterococcaceae were shared between airborne dust and three milk samples at another farm.
[Methods] Samples were collected in winter 2017 and in summer 2018 in two Jersey farms. Milk, feces, and blood samples were collected from 10 cows each. Feed, bedding, water, and airborne dust were also collected. Bacterial DNAs were purified and assessed by Miseq platform. Raw sequences were processed by QIIME 1 software. Obtained OTUs with 97% similarity were statistically analyzed using PRIMER 7 and JMP software.
[Results] Fecal microbiota were stable regardless of the farms and sampling times. Pseudomonadaceae, Lactobacillaceae, and Moraxellaceae were predominant in winter milk, whereas Pseudomonadaceae, Staphylococcaceae, and Lactobacillaceae were predominant in summer milk. Milk microbiota in winter was not related with fecal and cowshed microbiota but a relation with cowshed was shown in summer. Staphylococcaceae was also shared between airborne dust and two milk samples at one farm, and Pseudomonadaceae, Staphylococcaceae, and Enterococcaceae were shared between airborne dust and three milk samples at another farm.