15:10 〜 15:20
[VIII29-29E] Changes in Inflammatory Components of the Mammary Gland after Intrauterine Infusion of Lipopolysaccharide in Goats
This study was carried out to confirm the possibility of bacterial components translocation from the uterus to the mammary gland. Sixteen goats were divided into two groups: infused with 5 ml of saline with or without 100 µg of E. coli lipopolysaccharide (LPS), into the uterus. Milk and blood were collected at 0 - 144 h after infusion to measure the milk yield, somatic cell count (SCC) in milk, blood leukocyte count (BLC), cytokines and LPS-binding protein (LBP). Mammary gland tissues were collected before and at 24 h after infusion. There was no significant difference in milk yield, milk SCC between groups, but BLC was significantly decreased in LPS group. There was no significant difference in IL-1β, TNFα, IL-8, IL-10 concentration in plasma, however, IL-1β and IL-6 concentration in milk and LBP in plasma were was significantly higher in LPS than saline group. LPS was immunolocalized in the connective tissue and interepithelial spaces of alveoli of the mammary gland at 24 h after LPS infusion. These results suggested that intrauterine-infused LPS can be transferred to the mammary gland and induce the inflammatory responses on it.