繁殖・生殖工学

[IV-18-12] Expression and regulation of MMP3 in bovine endometrial cells in vitro

*Al-Nur Md. Iftekhar Rahman¹, Hayato Yamaguchi¹, Shutaro Horaku¹, Daichi Nishino¹, Qudratullah Qani¹, Toru Takahashi², Nobuhiko Yamauchi¹ (1. Kyushu Univ., 2. Iwate Univ.)

Matrix Metalloproteinase 3 (MMP3) plays an important role in endometrial remodeling as well as for successful implantation in bovine. There are few information for MMP3 production and its regulation in bovine endometrium till now. Therefore, this study aimed to characterize MMP3 expression in bovine endometrial cells in vitro. Bovine endometrial stroma (BES) and epithelial (BEE) cells were cultured without or with E2, P4 and IFNα (type-I interferon) for gene and zymographic analysis, respectively. The results of qPCR showed that gene expression of MMP3 was significantly high for E2 treated BES cells compared with the other groups. BEE cells had no effects in either of these treatments for MMP3 gene expression. Casein zymography showed that BES cells secrete MMP3 after 48 h of culture, where as BEE did not show any zymographic activity. Furthermore, MMP3 clearance was significantly high when BES was treated with E2. Secretion of MMP3 was significantly decreased when treated with P4 or IFNα. These results indicate that BES release MMP3 and the expression was regulated by E2, P4 and type-I interferon.