Best Presentation Award 4

[IVYS-06] Lipopolysaccharides infusion into the uterus causes inflammation in the mammary gland

〇Fika Yuliza Purba¹, Takahiro Nii², Yukinori Yoshimura², Naoki Isobe² (1.Graduate School of Biosphere Science, Hiroshima University, 2.Graduate School of Integrated Sciences for Life)

This study was carried out to demonstrate whether intrauterine-infused lipopolysaccharide (LPS) was translocated to the mammary gland, resulting in its inflammation. In Experiment 1, sixteen goats were intrauterine-infused with saline or LPS, while in Experiment 2, dexamethasone was administered before the infusion. Milk and blood were collected to measure the cytokines, acute-phase proteins, and antimicrobial peptides. In Experiment 1, the concentrations of IL-1β and IL-6 in milk were higher in the LPS group. In Experiment 2, somatic cell count was higher, whereas the milk yield and blood leukocyte count were lower in the LPS group than that in the control group. Lipopolysaccharide-binding protein and serum amyloid A in plasma, IL-1β, S100A8, and lactoferrin in milk were significantly higher in the LPS group. Taken together, these results suggest that LPS can be translocated from the uterus to the mammary glands and cause inflammation, especially under the immunosuppressive condition.