3. Reproduction/Reproductive technology

[P3-21] Effect of EAS on HSP70 related progesterone synthesis in bovine cumulus-granulosa cells

〇Khoi Thieu Ho¹, Kohei Homma², Jun Takanari², Tomohiro Mitani⁴, Hanako Bai¹, Manabu Kawahara¹, Kim Khang Thi Nguyen⁵, Masashi Takahashi³ (1.Graduate School of Agriculture, Hokkaido University, 2.AMINO UP Co. ltd., 3.Graduate school of Global Food Resources, Hokkaido University, 4.Field Science Center for Northern Biosphere, Hokkaido University, 5.College of Agriculture, Can Tho University, Can Tho, Viet Nam)

[Introduction] Bovine cumulus-granulosa (CG) cells has role for oocyte development and progesterone (P4) secretion. We have reported that a standardized extract of Asparagus officinalis stem (EAS) increase heat shock protein (HSP)70 expression under non heat shock condition in CG cells. However, effect of EAS on P4 synthesis in relation with HSP70-related regulation pathway is unclear. In this study, we investigated the effect of EAS on HSP70-related P4 synthesis in CG cells. [Materials and Methods] CG cells were cultured with 5 mg/ml of EAS for 6h and 12h at 38.5°C. Cells were collected for gene expression analysis for steroidogenesis-related genes (CYP11A1, 3β-HSD). Supernatant was collected for P4 level. EAS supplementation cells were treated with Pifithrin mu (PES), a HSP70 inhibitor, for 12h at 38.5°C. [Results] EAS significantly increased P4 level. 3β-HSD was significantly increased by EAS whereas no effect on CYP11A1. HSP70 inhibition by PES significantly decreased the EAS stimulated 3β-HSD, but it did not affect CYP11A1. These results suggest that EAS has useful effect on P4 synthesis in bovine CG cells via potential regulation of 3β-HSD pathway.