[P5-29] Establishment of new experimental model to investigate the mechanism by which water-soluble ZnPP is formed in Parma ham
[Objective] Zinc-protoporphyrin Ⅸ (ZnPP) is the predominant red pigment in Parma ham and is mainly water-soluble by binding to hemoglobin. In two experimental models we had established, only trace amount of water-soluble ZnPP was produced. In this study, we tried to establish a new model producing plenty of water-soluble ZnPP like Parma ham. [Methods] The porcine longissimus thoracis et lumborum (LTL) muscle was homogenized and then incubated anaerobically under different conditions. The fluorescence intensity of ZnPP was detected with spectrophotometer. ZnPP-binding proteins were determined with urea-PAGE and western blotting. [Results] In the established new model, the fluorescence intensity of water-soluble ZnPP increased 10-fold compared to previous experimental models by incubating 50% LTL muscle anaerobically at pH 5.5 for 10 days at 35℃. Urea-PAGE and western blotting showed that the ZnPP-binding protein was mainly hemoglobin same as Parma ham.