[IV-15-13] Relationship between milk Ig level and inflammation status induced by intramammary infusion of E.coli
[Objective] The aim of the present study was to examine relationship between immunoglobulin level in milk and inflammation status induced by intramammary infusion of E.coli in lactating goats.
[Materials and Methods] Goats (16 udders) were intramammary infused with 108 cells E.coli in 5 ml PBS at Day 0. Milk samples were collected every day from Day -2 to Day 7. At Day 0, milk was also collected at 2, 4, 6, 8 and 12 h after E.coli infusion. Milk yield, somatic cell count (SCC), Na+, total IgG, total IgA and E.coli-specific IgG concentrations in milk were measured.
[Results] Negative correlation was observed between E.coli-specific IgG and SCC at Day 1. Negative correlations were also observed between E.coli-specific IgG and Na+ at 8h at Day 0 and Day 4 whereas positive correlations were observed between total IgA and Na+ at 0 h, 2 h, Day 5, 6 and 7.
[Conclusion] These results suggest that higher milk concentration of E.coli-specific IgG resulted in weaker inflammation after bacterial stimulation.
[Materials and Methods] Goats (16 udders) were intramammary infused with 108 cells E.coli in 5 ml PBS at Day 0. Milk samples were collected every day from Day -2 to Day 7. At Day 0, milk was also collected at 2, 4, 6, 8 and 12 h after E.coli infusion. Milk yield, somatic cell count (SCC), Na+, total IgG, total IgA and E.coli-specific IgG concentrations in milk were measured.
[Results] Negative correlation was observed between E.coli-specific IgG and SCC at Day 1. Negative correlations were also observed between E.coli-specific IgG and Na+ at 8h at Day 0 and Day 4 whereas positive correlations were observed between total IgA and Na+ at 0 h, 2 h, Day 5, 6 and 7.
[Conclusion] These results suggest that higher milk concentration of E.coli-specific IgG resulted in weaker inflammation after bacterial stimulation.