畜産物利用2

[V-15-19] Characterization of immunobiotics for survivability in the GI tract

*Binghui Zhou^1,2,3, Yuhki Indo^1,2, Leonardo Albarracin^1,3,4, Yuki Masumizu^1,2, Md. Aminul Islam^1,2,3, Wakako Ikeda-Ohtsubo^1,2,3, Hisashi Aso^1,2,3, Julio Villena^1,3,4, Haruki Kitazawa^1,2,3 (1. Tohoku Univ. Agri., 2. CFAI, 3. C to C, 4. CERELA-CONICET)

[Purpose] In order to minimize the impact of antimicrobial drug resistance in livestock production, immunobiotics have been a possible candidate as antimicrobial substitutes due to the protective potentials of immunomodulatory effects against GI disorders. Immunobiotics need to be survived in the acidic gastric environment and bile salt if they are to reach the small intestine to exert health benefits through colonization. Here we investigated the survival potentials of immunobiotic Lactobacillus strains under bile salts and simulated gastric condition.
[Methods] Viable bacterial counts were measured following incubation of Lactobacillus strains incorporated with bile salt or the simulated gastric solution.
[Results] Immunobiotic Lactobacillus strains were able to survive in the gastric condition. Bile salt caused unrecoverable damage to cell bodies. The addition of wakame to lactobacilli had a protective effect on survival in gastric conditions. It is important for wakame assimilating immunobiotics to survive the GI tract. These results suggested the “immunosynbiotic” potentials of lactobacilli strain and wakame. [ACK] JRA