[V-15-25] Construction of genetically modified lactic acid bacteria producing anti-IL31Ra single-chain Fragment variable
[Objectives] In order to suggest the further application of lactic acid bacteria (LAB), we conducted research on genetically modified LAB (gmLAB). Here, we constructed a gmLAB producing anti-IL31 receptor A single-chain fragment variable (IL31RascFv) for atopic dermatitis treatment and evaluated the binding ability of the scFv. [Methods] The gene expression vector with IL31RascFv sequences was introduced into Lactococcus lactis NZ9000 to generate a gmLAB (designated as NZ-IL31RascFv). The expression analysis of IL31RascFv was performed using Western Blot (WB) and the immunoreactivity of scFv against antigen protein, IL-31 Ra, was investigated using ELISA. [Results] We confirmed a band corresponding to the IL31RascFv by WB. During ELISA, the absorbance increased in a concentration-dependent manner when NZ-IL31RascFv cellular extract was added to the well immobilized with IL-31 Ra. Therefore, we successfully constructed a gmLAB-expressing immunoreactive IL31RascFv.