2:50 PM - 3:00 PM
[V-16-27] Recombinant mouse interleukin 36 γ secreted by Lactococcus lactis induces Mucin2 mRNA expression in mouse colon cells
Microbial therapeutics have been researched for use in livestock animals. The intestinal mucus barrier prevents bacterial infections and maintains host-microbiota homeostasis. Recently, it has been reported that interleukin (IL) -36γ serves as a signal that induces mucin expression. We hypothesized that the delivery of IL-36γ to intestinal mucosa improves the gut environment. In this study, we constructed a genetically modified lactic acid bacteria (gmLAB) that secretes IL-36γ. The mouse (m) IL-36γ gene was inserted into the lactococcal secretion vector pNZ8148#2:SEC, and the resulting plasmid was introduced into Lactococcus lactis to generate a gmLAB designated NZ-IL36γ. The band corresponding to recombinant mIL-36γ was detected (by western blot) in the supernatant of NZ-IL36γ cultured with nisin. Next, we investigated whether mIL-36γ produced by NZ-IL36γ exhibits bioactivity in intestinal epithelial cells of a mouse colon cell line, CMT93/69; mIL-36γ purified from the gmLAB supernatant was compared to commercially available mIL-36γ. As a result, The addition of purified mIL-36γresulted accumulation of the Mucin 2.