[ODP1A] a. Phylogenetic Analyses, Taxonomy, and Typing

[ODP-001] Veillonella nakazawae sp. nov., isolated from the oral cavity of Japanese children

○Izumi Mashima^1,2, Citra F. Theodorea³, Futoshi Nakazawa³, Ariadna A. Djais³, Tadao Kunihiro⁴, Yoshiaki Kawamura², Masato Saitoh⁵, Maiko Otomo⁵, Riyoko Tamai¹, Yusuke Kiyoura¹ (¹Dept. Oral Med. Sci., Sch. Dent., Ohu Univ., ²Dept. Microbiol., Sch. Pharm., Aich Gakuin Univ., ³Dept. Oral Biol., Fac. Dent., Univ. Indonesia, ⁴Techno Suruga Labo., Co. Ltd., ⁵Dept. Ped. Dent., Sch. Dent., Heal. Sci Univ. Hokkaido)

Purpose: Recently, genus Veillonella have received a lot of attention to be a natural probiotic seed, because it was reported that they could utilize lactate in the human bodies for promoting the ability of exercise. Two strains of previously unknown gram-negative cocci belonging to genus Veillonella, T1-7^T and S6-16, were isolated from the oral cavity of healthy Japanese children. In this study, we have established these two strains as a novel Veillonella species based on the genomic, phylogenetic and phenotypic data.
Methods: After sequencing of 4 house keeping genes, phylogenetic analysis were performed. In addition, ANI and dDDH were calculated by using T1-7^T whole genome sequence data. Biochemical characteristics were also analyzed.
Results: Two strains showed the atypical catalase production for genus Veillonella. They are phylogenetically homogeneous and form a distinct, novel lineage within the genus Veillonella shared the highest similarities at 99.9%, 95.8%, 96.9%, and 96.7% using the partial 16S rRNA, dnaK, rpoB, and gltA gene sequences, respectively. Furthermore, strain T1-7^T shared the highest ANI (94.06%) and dDDH value (55.5%) with the most closely related species.
Conclusion: According to these observations, the two strains represent a novel species for which the name Veillonella nakazawae sp. nov. is proposed. The type strain is T1-7^T (JCM 33966^T = CCUG 74597^T).