[ODP-081] The dose-dependent positive effects of lincomycin on secondary metabolism in streptomycetes
Antibiotics are chemical compounds that suppress the growth of microorganisms. Meanwhile, antibiotics can act positively on bacteria. We have previously reported that ribosome-targeting antibiotics at concentrations below the minimum inhibitory concentration (MIC) profoundly enhanced secondary metabolism in actinomycetes, which are the major producer of bioactive compounds. However, the detailed mechanism of this phenomenon has not been clarified.
An antibiotic lincomycin (LIN) inhibits protein synthesis in susceptible bacteria by binding to the bacterial 50S ribosomal subunit. LIN strongly potentiates blue-pigmented antibiotic actinorhodin (ACT) production of the model actinomycetes Streptomyces coelicolor A3 (2). In this study, we investigated the dose-dependent effects of LIN on ribosome and its relationship with secondary metabolism in S. coelicolor A3 (2). Analysis of gene expression of ribosome-associated factors and ribosome profiles using sucrose density gradient revealed that ribosomes from cells treated with LIN at a concentration for enhanced ACT production changed qualitatively and quantitatively, which can cause efficient translation of ACT biosynthetic genes, resulting in high production of ACT.
Our findings suggest that LIN at concentrations below the MIC could cause positive changes in bacterial ribosomes, leading to enhanced secondary metabolism in actinomycetes.
An antibiotic lincomycin (LIN) inhibits protein synthesis in susceptible bacteria by binding to the bacterial 50S ribosomal subunit. LIN strongly potentiates blue-pigmented antibiotic actinorhodin (ACT) production of the model actinomycetes Streptomyces coelicolor A3 (2). In this study, we investigated the dose-dependent effects of LIN on ribosome and its relationship with secondary metabolism in S. coelicolor A3 (2). Analysis of gene expression of ribosome-associated factors and ribosome profiles using sucrose density gradient revealed that ribosomes from cells treated with LIN at a concentration for enhanced ACT production changed qualitatively and quantitatively, which can cause efficient translation of ACT biosynthetic genes, resulting in high production of ACT.
Our findings suggest that LIN at concentrations below the MIC could cause positive changes in bacterial ribosomes, leading to enhanced secondary metabolism in actinomycetes.