[ODP5B] b. 臨床微生物の検出・同定技術

[ODP-096] 敗血症性肺塞栓症患者から分離されたTsukamurella inchonensis の同定

○I Putu Bayu Mayura¹，Kazuyoshi Gotoh¹，美間 健彦¹，山本 由弥子¹，横田 憲治²，松下 治¹，萩谷 英大³ (¹岡山大・院・医歯薬・病原細菌，²岡山大・院・保健，³岡山大・院・医歯薬・総合内科)

Tsukamurella is an environmental, opportunistic pathogen that can cause pulmonary-, cutaneous infection, and meningitis. To date is difficult to distinguish Tsukamurella among related genera, e.g., Nocardia, Rhodococcus, and Gordonia due to sharing similar phenotypic properties, therefore molecular methods are necessary. In fact, 16S rRNA gene sequence analysis successfully distinguish Tsukamurella from another similar genus however, it uncertainly differentiates among Tsukamurella species. Thus, alternative gene targets, such as ssrA, secA, rpoB, and groEL were developed to correctly identified them. Herein, we encountered Tsukamurella inchonensis with septic pulmonary emboli (SPE), which is the first in the literature. A yellowish, rough, and fringed edges colony was detected as Gram-positive rods. However, matrix-assisted laser desorption/ionization-time of flight mass spectrometry failed to identify. The 16S rRNA gene sequence suggested the organism to be Tsukamurella species. In consequence of low resonance of species-level differentiation, we employed groEL, resulting in 100% matching (753/753 bps) with Tsukamurella inchonensis NCTC 10741 (GenBank accession no. LR131273.1), which has been incorrectly registered in the database as a Tsukamurella paurometabola. Among Tsukamurella infection molecular diagnosis of the pathogens requires for identification of species.