[ODP-101] 北日本の北海道におけるスタフィロコッカス・アルジェンテウスの分子疫学
Background: Staphylococcus argenteus is a novel staphylococcal species which is genetically close to S. aureus. This study was conducted to determine prevalence and genetic characteristics of S. argenteus in clinical isolates in Hokkaido, Japan.
Methods: S. argenteus was screened by MALDI-TOF, and genotypes and the presence of virulence factors/drug resistance genes were analyzed by PCR and sequencing. MIC to various drugs was examined by broth microdilution test.
Results: S. argenteus obtained was 82 isolates, which corresponded to 0.66% of total number of S. aureus isolates in the same period. They were classified into 3 genotypes, ST2250-coa-XId (37, 45.1%), ST1223-coa-XV (25, 30.5%), and ST2198-coa-XIV (20, 24.4%). All the ST1223 isolates had egc-2 cluster and 60% of isolates harbored seb. All the ST2250 isolates had sey, and 68% of this ST carried sel26-sel27. Some of ST2250 isolates had sec3 and tst-1 gene while an ST2198 isolate had sea2. Panton-Valentine leucocidin was detected only in an ST2250 isolate. Only an ST2250 isolate had mecA in SCCmec-IVc. Compared with other STs, various drug resistance genes (blaZ, erm (C) , msr (A) , aac (6’)-Ie-aph (2’’)-Ia, and lnuA) were identified mostly in ST2198 clones.
Conclusion: Prevalence of drug resistance genes and virulence factors was different depending on STs of S. argenteus.
Methods: S. argenteus was screened by MALDI-TOF, and genotypes and the presence of virulence factors/drug resistance genes were analyzed by PCR and sequencing. MIC to various drugs was examined by broth microdilution test.
Results: S. argenteus obtained was 82 isolates, which corresponded to 0.66% of total number of S. aureus isolates in the same period. They were classified into 3 genotypes, ST2250-coa-XId (37, 45.1%), ST1223-coa-XV (25, 30.5%), and ST2198-coa-XIV (20, 24.4%). All the ST1223 isolates had egc-2 cluster and 60% of isolates harbored seb. All the ST2250 isolates had sey, and 68% of this ST carried sel26-sel27. Some of ST2250 isolates had sec3 and tst-1 gene while an ST2198 isolate had sea2. Panton-Valentine leucocidin was detected only in an ST2250 isolate. Only an ST2250 isolate had mecA in SCCmec-IVc. Compared with other STs, various drug resistance genes (blaZ, erm (C) , msr (A) , aac (6’)-Ie-aph (2’’)-Ia, and lnuA) were identified mostly in ST2198 clones.
Conclusion: Prevalence of drug resistance genes and virulence factors was different depending on STs of S. argenteus.