[ODP-175/WS9-1] ヒトiPS細胞由来腸管上皮細胞の特性と腸内細菌機能評価系への応用
Purpose: Gut microbiota involve biological functions, including obesity, depression, and intestinal immunity. To clarify the intestinal bacterial functions and their mechanisms, an evaluation model with the human intestinal tract is required. So, our objective is to establish in vitro human intestinal model to evaluate the reactivity of commensal bacteria.
Method: We modified the previous report (Iwao et al., 2015) and established intestinal epithelial cells differentiated from human iPS cells (F-hiSIECTM). The characteristics of F-hiSIEC were compared to human small intestine and Caco-2 cells. We also measured the gene expressions in response to Toll-like-receptor (TLR) ligands or commensal bacterial metabolites.
Result: F-hiSIEC showed the comparable mRNA expression levels of intestinal epithelial cell markers, metabolic enzymes, and transporters with human adult small intestine. In point of immune response, TLR1-5 and GPRs, the receptors of short chain fatty acids (SCFAs), were expressed in F-hiSIEC. Moreover, these cells showed that the gene expression of pro-inflammatory cytokine was elevated in response to TLR ligands. On the other hand, expression levels of anti-inflammatory cytokines were increased in response to SCFAs.
Conclusion: F-hiSIEC will be a useful in vitro system to evaluate the intestinal immunity and interaction with gut microbiota.
Method: We modified the previous report (Iwao et al., 2015) and established intestinal epithelial cells differentiated from human iPS cells (F-hiSIECTM). The characteristics of F-hiSIEC were compared to human small intestine and Caco-2 cells. We also measured the gene expressions in response to Toll-like-receptor (TLR) ligands or commensal bacterial metabolites.
Result: F-hiSIEC showed the comparable mRNA expression levels of intestinal epithelial cell markers, metabolic enzymes, and transporters with human adult small intestine. In point of immune response, TLR1-5 and GPRs, the receptors of short chain fatty acids (SCFAs), were expressed in F-hiSIEC. Moreover, these cells showed that the gene expression of pro-inflammatory cytokine was elevated in response to TLR ligands. On the other hand, expression levels of anti-inflammatory cytokines were increased in response to SCFAs.
Conclusion: F-hiSIEC will be a useful in vitro system to evaluate the intestinal immunity and interaction with gut microbiota.