[ODP-203] 活性イオウドナーによるマクロファージおよび好中球依存性殺菌作用の増強効果
Background: Cysteine persulfide and polysulfides are cysteine derivatives having sulfane sulfur atoms bound to cysteine thiol. Accumulating evidence has suggested that cysteine persulfides/polysulfides are abundant in prokaryotes and eukaryotes and play important roles in diverse biological processes. Here, we examined the effects of chemically synthesized polysulfide donors on antibacterial activity of macrophages and neutrophils.
Methods: N-Acetyl-l-cysteine (NAC) connecting with polysulfur chains were synthesized as novel polysulfide donors, called NAC polysulfides. Macrophages or neutrophils were infected with Escherichia coli, Salmonella enterica serovar Typhimurium LT2, or Staphylococcus aureus. Gentamycin was applied to kill extracellular bacteria for overnight culture. NAC polysulfides were added into infected-cells during gentamycin protection assay.
Results: Treatment of cells with NAC polysulfides remarkably decreased intracellular bacterial numbers. NAC polysulfides-enhanced bacterial killing in macrophages was not due to nitric oxide and cytokine production, as well, reactive oxygen species generation.
Conclusion: Precise understanding of mechanisms by which NAC polysulfides-mediated bacterial killing in macrophages and neutrophils may be helpful to develop therapeutic strategies for bacterial infectious diseases.
Methods: N-Acetyl-l-cysteine (NAC) connecting with polysulfur chains were synthesized as novel polysulfide donors, called NAC polysulfides. Macrophages or neutrophils were infected with Escherichia coli, Salmonella enterica serovar Typhimurium LT2, or Staphylococcus aureus. Gentamycin was applied to kill extracellular bacteria for overnight culture. NAC polysulfides were added into infected-cells during gentamycin protection assay.
Results: Treatment of cells with NAC polysulfides remarkably decreased intracellular bacterial numbers. NAC polysulfides-enhanced bacterial killing in macrophages was not due to nitric oxide and cytokine production, as well, reactive oxygen species generation.
Conclusion: Precise understanding of mechanisms by which NAC polysulfides-mediated bacterial killing in macrophages and neutrophils may be helpful to develop therapeutic strategies for bacterial infectious diseases.