[ODP7B] b. 薬剤耐性

[ODP-205] 国内で分離されたクロノバクター属菌の病原遺伝子保有状況及び薬剤耐性

○岡田 由美子¹，中山 達哉¹，大竹 正悟²，笠井 正志²，鈴木 穂高³，荻原 博和⁴，Stephen James Forsythe⁵ (¹国立衛研・食品衛生管理，²兵庫県立こども病院・感染症内科，³茨大・農，⁴日大・生物資源科学，⁵foodmicrobe.com)

Cronobacter spp. cause meningitis and bacteremia in infants and immunocompromised adults. In this study, prevalence of virulence associated genes and antibiotic resistance patterns of Cronobacter spp. isolated in Japan were determined. Seven types of virulence associated genes (ompA, inv, hly, cpa, sip, aut, fliC) were screened by PCR, using 17 Cronobacter spp. isolates; 1 from patient, 7 from bovine faeces, 9 from foods retailed in Japan. Antibiotic resistance against ampicillin, cefotaxime, ceftazidime, cefoxitin, meropenem, nalidixic acid, ciprofloxacin, trimethoprim/sulfamethoxazole, gentamicin, kanamycin, streptomycin, tetracycline, chloramphenicol, and fosfomycin were determined by disc diffusion method. All isolates were positive for fliC, encoding flagellin, and the most isolates were positive for ompA, encoding outer membrane protein. All isolates were negative for sip, encoding siderophore-interacting protein, and aut, a putative virulence factor. Twelve out of 17 (70.6%) isolates including one clinical isolate showed resistance to fosfomycin. Almost all isolates were susceptible or intermediate to all antibiotics used in this study except fosfomycin, whereas 2 isolates from bovine faeces showed resistance to the other antibiotics. These results suggest that the situation of antibiotic resistance of Cronobacter isolates in Japan is not critical at present.