[ODP7C] c. その他

[ODP-215] 光抗菌療法の開発

○佐藤 和秀^1,2,3,4，安井 裕智³，高橋 一臣³，滝 俊一³，紅 朋浩⁵，中川 善之⁵ (¹名古屋大学高等研究院，²最先端イメージング分析センター／医工連携ユニット，³名古屋大学大学院医学系研究科病態内科学講座呼吸器内科学，⁴名古屋大学未来社会創造機構，⁵医真菌・オミクス講座)

Purpose: In recent years, resistant bacteria are becoming more and more difficult to treat in clinical practice, and it is desirable to develop a treatment method that is different from conventional concepts. To address this issue, we are developing targeted photo-antibacterial targeting therapy (PAT2) using near-infrared light.
Methods: Chickens were immunized with Candida albicans antigen and anti-Candida albicans IgY (CA-IgY) was produced. Then, IgY was conjugated with IR700 to prepare CA-IgY-IR700. Binding of human skin cancer cells, A431 and C.albicans related strains, to CA-IgY-IR700 was assessed. The effect of PAT2 in vitro was examined with microscopy. We also examined the effects of PAT2 in vivo using a mouse skin ulcer infection model.
Results: CA-IgY-IR700 did not bind to human cells but was widely bound to the Candida family. The effect of PAT2 in vitro was increased in a dose-dependent manner for NIR-light. PAT2 was found to have a rapid cell death effect; in vivo PAT2 was found to have a marked infection ulcer healing effect and was also found to be an appropriate targeted treatment without causing extra inflammation from treatment.
Conclusions: PAT2 with CA-IgY-IR700 was considered a highly selective and promising antimicrobial therapy.