The 94th Annual Meeting of Japanese Society for Bacteriology

Presentation information

Workshop

[WS4] Selected from Oral Session: Microbial Taxonomy, Physiology, Structure and Ecology / Application of Microorganisms

Tue. Mar 23, 2021 4:00 PM - 6:00 PM Channel 4

Conveners: Tsutomu Sekizaki (The University of Tokyo), Hideaki Nagamune (Tokushima University)

[WS4-8/ODP-018] Shotgun metagenome sequencing identification of microbial genes associated with an oral disease

○Koji Yahara4, Hiroko Yahara1, Akimitsu Hiraki2, Yutaka Maruoka3, Aki Hirabayashi4, Masato Suzuki4 (1Genome Med. Sci. P.J. (Toyama), R.I., NCGM, 2Sec. Oral Oncol., Dep. Oral Maxillofac. Surg, Fukuoka Dental. Coll., 3Dep. Oral Maxillofac. Surg, H.P., NCGM, 4AMR Res. Ctr., NIID)

Medication-related osteonecrosis of the jaw (MRONJ) is an oral disease in which infection is suggested. Prior studies that explored the association between MRONJ and microbial infection were limited to the culture-based approach or 16S ribosomal RNA gene sequencing. Here, deep shotgun metagenome sequencing (>10 Gb per sample) of bulk DNA extracted from saliva of MRONJ patients and healthy controls was performed to overcome these limitations. Comparative quantitative analyses of these deep metagenomes revealed an average 10.1% increase of genus Actinomyces and a 33.2% decrease in genus Streptococcus. Pan-genome analysis identified genes present exclusively in the MRONJ samples. Further analysis of the reads mapping to the genes in an extended dataset resulted in the identification of 31 genes significantly associated with MRONJ. All these genes were encoded by Actinomyces genomic regions. Of these, the top two abundant genes were almost exclusively encoded by Actinomyces among usual taxa in the human oral microbiota. The potential relationships of these key genes with the disease are discussed at molecular level based on the literature. Although the sample size was small, this study will aid future studies to verify the data and characterize these genes to understand the disease mechanisms, develop molecular targeted drugs, and for early stage screening (PLOS One, in press).