The 94th Annual Meeting of Japanese Society for Bacteriology

Presentation information

Workshop

[WS9] Selected from Oral Session: Virulence Factors and Biophylaxis: Infection Models, Parasitism, Immunity, Vaccines / Pathogens and Infectious Diseases

Thu. Mar 25, 2021 12:45 PM - 2:45 PM Channel 3

Conveners: Yukako Fujinaga (Kanazawa University), Tomoko Sumitomo (Osaka University)

[WS9-1/ODP-175] In vitro Bacterial Evaluation Model using Human iPS Cell-derived Small Intestinal Epithelial Cells

○Nao Yamazaki1, Shinji Mima1, Yuki Imakura1, Takahiro Iwao2, Tamihide Matsunaga2, Shinichi Watanabe1, Kozo Nagata1, Masahiko Taniguchi1 (1Bio Science & Engineering Laboratory, FUJIFILM Corporation, 2Dept. Clinical Pharmacy, Grad. Sch. Pharmaceutical Sciences, Nagoya City Univ.)

Purpose: Gut microbiota involve biological functions, including obesity, depression, and intestinal immunity. To clarify the intestinal bacterial functions and their mechanisms, an evaluation model with the human intestinal tract is required. So, our objective is to establish in vitro human intestinal model to evaluate the reactivity of commensal bacteria.
Method: We modified the previous report (Iwao et al., 2015) and established intestinal epithelial cells differentiated from human iPS cells (F-hiSIECTM). The characteristics of F-hiSIEC were compared to human small intestine and Caco-2 cells. We also measured the gene expressions in response to Toll-like-receptor (TLR) ligands or commensal bacterial metabolites.
Result: F-hiSIEC showed the comparable mRNA expression levels of intestinal epithelial cell markers, metabolic enzymes, and transporters with human adult small intestine. In point of immune response, TLR1-5 and GPRs, the receptors of short chain fatty acids (SCFAs), were expressed in F-hiSIEC. Moreover, these cells showed that the gene expression of pro-inflammatory cytokine was elevated in response to TLR ligands. On the other hand, expression levels of anti-inflammatory cytokines were increased in response to SCFAs.
Conclusion: F-hiSIEC will be a useful in vitro system to evaluate the intestinal immunity and interaction with gut microbiota.