[ODP-012] Molecular epidemiological properties of CPILE-producing Clostridium perfringens by MLST analyses
Clstririum perfringens iota-like enterotoxin (CPILE) is identified as a new enterotoxin of foodborne pathogen producing C. perfringens.
The CPILE-producing C. perfringens strains isolated from many various samples were investigated by molecular epidemiological analyses.
Materials and methods: Fourteen strains of CPILE-producing C. perfringens, 7 CPE producing C. perfringens serotype TW27, and 11 C. perfringens serotype TW27 were examined. Strains were isolated from feces of the patients, feces of healthy food handlers, food, and animal samples collected from 1997 to 2019. The strains were undertaken to serotyping, PFGE analysis, and MLST analysis.
Results and Conclusions: Four strains of fourteen CPILE-producing strains belonged to serotype TW27. The other strains were Hobbs5, TW21, 81, 84, 86 (2 strains) 88, 90 and untypable serotype. PFGE patterns of four CPILE-producing strains with serotype TW27 were distinguishable from each other. In MLST analysis, all of the closest CPILE-producing strains of serotype TW27 were in ST 20, were classified into the same cluster as C. perfringens serotype 27 (non-CPE producing).The nearest STs of CPILE-producing strains other than the serotype TW27 strains were ST131 (3 strains), 177 (3 strains), 131 and 142 (2 strains), 142 (1 strain) and 95 (1 strain).
Our findings suggested CPILE-producing C. perfringens were classified into several clusters.