[ODP2] 1. 分類・疫学・感染症-b. 疫学・分子疫学

[ODP-012] 新型下痢原性毒素CPILE産生性ウェルシュ菌の分子疫学解析

門間 千枝¹，岡田 若葉¹，赤瀬 悟¹，横山 敬子¹，貞升 健志¹，鎌田 洋一² (¹東京都健安研・微生物，²千里金蘭大学)

Clstririum perfringens iota-like enterotoxin (CPILE) is identified as a new enterotoxin of foodborne pathogen producing C. perfringens.
The CPILE-producing C. perfringens strains isolated from many various samples were investigated by molecular epidemiological analyses.
Materials and methods: Fourteen strains of CPILE-producing C. perfringens, 7 CPE producing C. perfringens serotype TW27, and 11 C. perfringens serotype TW27 were examined. Strains were isolated from feces of the patients, feces of healthy food handlers, food, and animal samples collected from 1997 to 2019. The strains were undertaken to serotyping, PFGE analysis, and MLST analysis.
Results and Conclusions: Four strains of fourteen CPILE-producing strains belonged to serotype TW27. The other strains were Hobbs5, TW21, 81, 84, 86 (2 strains) 88, 90 and untypable serotype. PFGE patterns of four CPILE-producing strains with serotype TW27 were distinguishable from each other. In MLST analysis, all of the closest CPILE-producing strains of serotype TW27 were in ST 20, were classified into the same cluster as C. perfringens serotype 27 (non-CPE producing).The nearest STs of CPILE-producing strains other than the serotype TW27 strains were ST131 (3 strains), 177 (3 strains), 131 and 142 (2 strains), 142 (1 strain) and 95 (1 strain).
Our findings suggested CPILE-producing C. perfringens were classified into several clusters.