[ODP16] 4. Genetics / Genomics / Biotechnology -a. Genomics, bioinformatics and systems biology

[ODP-077] The discovery and evaluation of the novel energy metabolic pathway in oral Veillonella

Izumi Mashima¹, Yu-Chieh Liao², Chieh-Hua Lin², Futoshi Nakazawa³, Elaine Haase⁴, Yusuke Kiyoura¹, Frank Scannapieco⁴ (¹Dept. Oral Med. Sci., Sch. Dent., Ohu Univ., ²Inst. Pop. Heal. Sci., Nat. Heal. Res. Inst., ³Dept. Oral Biol., Fac. Dent., Univ. Indonesia, ⁴Dept. Oral Biol., Sch. Dent. Med., Univ. Buffalo, SUNY)

Purpose: Genus Veillonella have enough possibilities to be an oral probiotic seed, because they can utilize lactate as their energy source which has been reported the cause of dental caries. However, the overall their metabolic pathways have not been clarified.
To proceed the probiotic studies of oral Veillonella, the pan-genome analysis was investigated. In this presentation, we focused on the pathway of fructose consumption as a novel energy metabolic function in all oral Veillonella.
Methods: Pan-genome analysis was performed by BPGA pipeline using the gbk files of all oral Veillonella genomes. The core, accessory and unique gene families identified by the BPGA pipeline were then used to perform KEGG pathways. The growth curves of oral Veillonella spp. with fructose substrates were also investigated in this study.
Results: The fructose metabolic pathways were constructed by core genomes, meaning that it was conserved in all oral Veillonella. Furthermore, it was confirmed that oral Veillonella could also grow with fructose substrates only.
Conclusion: These results proved for the first time that oral Veillonella had the ability to consume the fructose as an energy source. We are planning to develop the oral probiotic seeds with genome editing focused on “anti-caries” to downregulate this pathway of fructose consumption, and then to enhance lactate consumption.